Antibody Identity card
Antibody information [TOX (Thymocyte Selection Associated High Mobility Group Box)]
EP12540
- Clone name EP12540
- Description Rabbit monoclonal
- Antigen used Recombinant fragment (information not available)
- Epitope Unknown
- Isotype IgG
- Confirmed species reactivity Human
- Ab used Abcam ab181197 (purified mAb 0.321 mg/ml)
| APPLICATION | Recommended concentration | Status | Protocol |
|---|---|---|---|
| Western blotting (WB) | 2.5 ug/ml | Not working | Odyssey Western Blotting protocol (OdWB) |
| Immunocytochemistry (ICC) | 2.5 μg/ml | Not working | ICC in frozen tissue and cytospin preparation |
| Immunohistochemistry (IHC-P) | 2.5 μg/ml | Not working | BOND-MAX automated immunohistochemistry |
| Immunoflourescence (IF) | 2.5 μg/ml | Not working | Immunofluorescence staining |
| Flow cytometry (FC) | 2.5 μg/ml | Not working | Flow cytometry |
| IHC-P Species | 2.5 μg/ml | Not working | BOND-MAX automated immunohistochemistry |
Ab ID Card application validation / characterisation
WB Validation
Over-expression/cross reactivity
Not working
Gene inactivation
Not working
WB Characterisation
Endogenous expression
Not working
ICC / ICC-P Validation
Over-expression/cross reactivity
Not working
Gene inactivation
Not working
IHC-P Characterisation
Endogenous expression
Not working
IF Characterisation
Endogenous expression
Not tested
FC Validation
Over-expression/cross reactivity
Not working
Gene inactivation
Not working
FC Characterisation
Endogenous expression
Not working
IHC-P Domestic species
Not tested
IHC-P Wild species
Not tested