mAbs Against MOUSE Protein
BCL6
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse anti mouse |
| CLONE NAME: | ST42B |
| PROTEIN: | human full length Bcl-6 |
| PROTEIN WEB: | http://www.hprd.org/summary?hprd_id=00180&isoform_id=00180_3&isoform_name=Isoform_1 |
| ANTIGEN USED: | GST-BCL6 recombinant protein |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human and mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| APP RECOMMENDED: | IHQ-frozen, IHQ-paraffin, WB, IF |
| APP NO TESTED: | Flow Cytometry, IP |
Description
BCL-6 is a transcriptional regulator gene which codes for a 706-amino-acid nuclear zinc finger protein. Antibodies to this protein stain the germinal centre cells in lymphoid follicles, the follicular cells and interfollicular cells in Follicular Lymphoma, Diffuse Large B-Cell Lymphomas, and Burkitt’s lymphoma, and the majority of the Reed-Sternberg cells in Nodular Lymphocyte Predominant Hodgkin’s Disease. In contrast, anti-BCL-6 rarely stains Mantle Cell Lymphoma and MALT Lymphoma. BCL-6 expression is seen in approximately 45% of CD30+ Anaplastic Large Cell Lymphoma but is consistently absent in other peripheral T-cell lymphomas.
Publication describing antibody characterization/validation
García JF, García JF, Maestre L, Lucas E, Sánchez-Verde L, Romero-Chala S, Piris MA, Roncador G. Genetic Immunization: a New Monoclonal Antibody for the Detection of BCL-6 Protein in Paraffin Sections. J Histochem Cytochem. 2006 54(1):31-8. http://www.ncbi.nlm.nih.gov/pubmed/16046671
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | ST42B | 1:30 | purified | Discovery Xt CC1 OmniMap | Ventana | lymph node / | nuclear | human | |
| Immunofluorescence | |||||||||
Enlarge image
- 42B mAb staining in mouse lymph node
- In lymph node, BCL6 protein is strongly expressed by B cells.
Enlarge image
- ST42B expression in one mouse lymphoma paraffin section.