mAbs Against MOUSE Protein
BCL6
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse anti mouse |
| CLONE NAME: | GI191E |
| PROTEIN: | human full length BCL6 |
| PROTEIN WEB: | http://www.hprd.org/summary?hprd_id=00180&isoform_id=00180_3&isoform_name=Isoform_1 |
| ANTIGEN USED: | pcDNA3-BCL6 |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | human and mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| APP RECOMMENDED: | WB, IHC-frozen, IHC-paraffin |
| APP NO TESTED: | IP, IF, Flow Cytometry |
Description
BCL-6 is a transcriptional regulator gene which codes for a 706-amino-acid nuclear zinc finger protein. Antibodies to this protein stain the germinal centre cells in lymphoid follicles, the follicular cells and interfollicular cells in Follicular Lymphoma, Diffuse Large B-Cell Lymphomas, and Burkitt’s lymphoma, and the majority of the Reed-Sternberg cells in Nodular Lymphocyte Predominant Hodgkin’s Disease. In contrast, anti-BCL-6 rarely stains Mantle Cell Lymphoma and MALT Lymphoma. BCL-6 expression is seen in approximately 45% of CD30+ Anaplastic Large Cell Lymphoma but is consistently absent in other peripheral T-cell lymphomas.
Publication describing antibody characterization/validation
García JF, García JF, Maestre L, Lucas E, Sánchez-Verde L, Romero-Chala S, Piris MA, Roncador G. Genetic Immunization: a New Monoclonal Antibody for the Detection of BCL-6 Protein in Paraffin Sections. J Histochem Cytochem. 2006 54(1):31-8. http://www.ncbi.nlm.nih.gov/pubmed/16046671
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | GI191E | neat | supernatant | Discovery Xt CC1 OmniMap | Ventana | Lymph node / | nuclear | human | |
| Immunofluorescence | |||||||||
Enlarge image
- MAb IG191E in mouse tissue
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | GI191E | supernatant | 1:20 | WR19L cell line / Ba/F3 cell line | 78kDa | 80kDa | human | Available |
| Immunoprecipitation | ||||||||
Enlarge image
- Western Blotting of BCL6 (GI191E)
- Western Blotting of BCL6 (GI191E) using different mouse and rat cell lines.
Lane 1 Ba/F3 mouse cell line (10ug) (-)
Lane 2 WR19L mouse cell line (50ug) (+)
Lane 3 PC12 rat cell line (50ug) (+)