mAbs Against MOUSE Protein
CDK6
| STATUS: |  |
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| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
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| STATUS: | Validated |
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| TYPE: | mouse anti mouse |
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| CLONE NAME: | 98D |
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| PROTEIN: | Cyclin dependent kinase 6 |
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| PROTEIN WEB: | http://www.ncbi.nlm.nih.gov/protein/AAD43504.1 |
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| ANTIGEN USED: | His-mCDK6 recombinant protein (full length) |
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| FUSION PARTNER: | myeloma p3-NS1/Ag4-1 (NS1) cell |
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| ISOTYPE: | IgG1 |
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| SPECIES REACTIVITY: | human and mouse |
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| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
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Applications
IHC Techniques
| IHC Techniques |
Clone |
Dilution |
Antibody concentration |
Antigen retrieval method |
Visualization kit |
+/- control |
Protein localization |
Positivity in other species |
Protocol |
| Frozen tissue and cytospins |
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| Paraffin tissue |
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Recommended Result obtained is satisfactory. The reagent can be use in this application | 98D |
neat |
supernatant |
Discovery Xt Std RiboCC |
Ventana |
lymph node / |
|
mouse |
|
| Immunofluorescence |
|---|
WB Techniques
| WB Techniques |
Clone |
Dilution |
Antibody concentration |
+/- control |
Expected MW |
Observed Mw |
Positivity in other species |
Protocol |
| Western Blotting |
|---|
Recommended Result obtained is satisfactory. The reagent can be use in this application | 98D |
|
supernatant |
3T3 cell line / |
37kDa |
37kDa |
mouse |
Available |
| Immunoprecipitation |
|---|
Enlarge image
- Western Blotting of CDK6 (98D) using different mouse cell and tissues extracts
- Lane 1 RG15WT (200ug)
Lane 2 RG17KO (200ug)
Lane 3 3T3 cell line (200ug)
Enlarge image