mAbs Against MOUSE Protein
FRA2
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | Rat anti mouse |
| CLONE NAME: | REY146C |
| PROTEIN: | Fos like antigen 2 |
| PROTEIN WEB: | http://www.ncbi.nlm.nih.gov/nucleotide/229577213?report=genbank&log$=nuclalign&blast_rank=1&RID=ZNM5H8A201R |
| ANTIGEN USED: | HIS-mFRA2 (178-326aa) recombinant protein |
| FUSION PARTNER: | myeloma p3-NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG2a |
| SPECIES REACTIVITY: | mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
References
Wurm S, Zhang J, Guinea-Viniegra J, García F, Muñoz J, Bakiri L, Ezhkova E, Wagner EF. Terminal epidermal differentiation is regulated by the interaction of Fra-2/AP-1 with Ezh2 and ERK1/2. Genes dev.2015 Jan 15;29 (2):144-56.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | REY146C | Neat supernatant | / | ||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | REY146C | 1:50 | / | ||||||
| Immunofluorescence | |||||||||
Enlarge image
- REY146C mAb in HEK293T transfected cells
- To confirm that REY146C mAb recognized mouse FRA2 protein, immunohistochemistry on frozen cytospins preparations of V5-tagged mFRA2 expressed in Hek293T was performed. Cytospin preparation of mouse FRA1 and mouse FOSB transfected cells was used as negative controls to confirm the specificity of the antibody. Labeling with the anti-V5, anti-FOSB and anti-FRA1 antibodies confirmed the efficiency of the transfections.
Enlarge image
- REY146C antibody can be used to detect mouse FRA2 protein in paraffin embedded tissue samples.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | REY146C | 1:100 | Supernatant | / | Available | |||
| Immunoprecipitation | ||||||||
Enlarge image
- REY146C mAb is able to detect mFRA2 protein by WB.
- Lane 1 Mouse keratinocytes wt (100ug)
Lane 2 Mouse keratinocytes FRA2 KO (100ug)
Lane 3 Mouse epidermis wt (100ug)
Lane 4 Mouse epidermis FRA2 KO (100ug)