mAbs Against MOUSE Protein
HIF1A
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | Rat monoclonal |
| CLONE NAME: | SIMA343B |
| PROTEIN: | Hypoxia-inducible factor 1-alpha |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/Q61221 |
| ANTIGEN USED: | mouse HIS-HIF1a (fragment 530-825) |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG2a |
| SPECIES REACTIVITY: | human and mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | SIMA343B | Neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | SIMA343B | 1:30 | supernatant | Placenta / Liver | |||||
| Immunofluorescence | |||||||||
Enlarge image
- SIMA343B mAb is able to detect human and mouse HIF1a protein in immunocytochemistry
- To confirm that SIMA343B mAb recognizes human and mouse HIF1a protein, immunocytochemistry on frozen cytospins preparation of human and mouse HIF1a and hHIF2a expressed in HEK293T was performed. Anti-MYC, anti-HA and anti GFP Abs were used as positive controls. Cytospin preparation of mouse PDL1 protein was used as negative control.
Enlarge image
- SIMA343B mAb is able to detect mouse HIF1a protein in mouse paraffin tissues
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | SIMA343B | Neat | supernatant | / | 93kDa | >100kDa | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- SIMA343B mAb is able to detect human Hif1a protein by WB (over-expression).
- Predicted molecular weight: 93kDa
Observed molecular weight: >100kDa
LANES
Lane 1 HEK-mouse HIF1A-MYC-DDK (10ug) (+)
Lane 2 HEK-human HIF1A-HA (10ug) (+)
Lane 3 HEK-human HIF2a-GFP (10ug) (-)