mAbs Against MOUSE Protein
MCMV IE1
| STATUS: | |
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| CONTACT INFORMATION: | Center for Proteomics, University of Rijeka |
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| TYPE: | monoclonal |
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| CLONE NAME: | IE1.01 |
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| PROTEIN: | MCMV immediate early protein 1 |
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| PROTEIN WEB: | http://www.ncbi.nlm.nih.gov/protein/AAA74504.1 |
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| ANTIGEN USED: | lysates of MCMV infected cells |
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| FUSION PARTNER: | SP2O |
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| ISOTYPE: | kappa IgG2a |
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| SPECIES REACTIVITY: | MCMV |
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| PREPARATION AND STORAGE: | affinity purified, dilluted in PBS, -20C long term, +4 short term, no azide added |
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Description
Specificity of the antibody was confirmed by mass spectrometry analysis of immunoprecipitated protein using this mAb.
Applications
IHC Techniques
| IHC Techniques |
Clone |
Dilution |
Antibody concentration |
Antigen retrieval method |
Visualization kit |
+/- control |
Protein localization |
Positivity in other species |
Protocol |
| Frozen tissue and cytospins |
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| Paraffin tissue |
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| IE1.01 |
1:1000 |
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|
|
MCMV infected tissue / uninfected tissue |
nuclear |
|
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| Immunofluorescence |
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| IE1.01 |
|
|
|
FITC |
MCMV infected cells / uninfected cells |
nuclear |
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Enlarge image
- FLuorescence IE1.01
- Murine embryonal fibroblasts (MEFs) from Balb/c mice were infected with Smith strain of MCMV.
Enlarge image