mAbs Against MOUSE Protein
NGFR
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | Rat monoclonal |
| CLONE NAME: | NORI146C |
| PROTEIN: | NGFR Tumor necrosis factor receptor superfamily member 16 |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/Q9Z0W1 |
| ANTIGEN USED: | His-mNGFR (276-427aa) protein |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG2a |
| SPECIES REACTIVITY: | mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
ow affinity neurotrophin receptor which can bind to mature NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF (proNGF), BDNF (proBDNF) and NTF3 (proNT3) with high affinity, and has much lower affinity for mature NGF and BDNF. Plays an important role in differentiation and survival of specific neuronal populations during development. Can mediate cell survival as well as cell death of neural cells. The heterodimeric receptor formed with SORCS2 plays a role in proBDNF-dependent synaptic plasticity, in hippocampal long term depression (LTD) and long term potentiation (LTP). Plays a role in the inactivation of RHOA (By similarity). Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | NORI146C | Neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | NORI146C | 1:200 | supernatant | / | |||||
| Immunofluorescence | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | NORI146C | 1:1 | supernatant | / | Available | ||||
Enlarge image
- NORI146C mAb is able to detect mouse NGFR protein in immunocytochemistry.
- To confirm that NORI146C mAb recognizes mouse NGFR protein, immunocytochemistry on frozen cytospins preparations of MYC-tagged mNGFR expressed in HEK293 was performed. Anti-MYC was used as positive control. Cytospin of HEK293 transfected with mouse HIF1a were used as a negative control.
Enlarge image
- NORI146C mAb is able to detect mouse NGFR protein by immunohistochemistry in paraffin samples.
- Paraffin samples of NGFR KO mouse model B6.129S4-Ngfrtm1Jae/6J.OlaHsd
Enlarge image
- NORI146C mAb is able to detect mouse NGFR protein by immunofluorescence in tissue samples
- TISSUE SAMPLE: Dorsal root ganglions of C57BL/6J.OlaHsd E10.5 Mouse embryo
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | NORI146C | Neat | supernatant | / | 45 | 60 | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- NORI146C mAb is able to detect mouse NGFR protein by WB.
- LANES
Lane 1 Melanoma Cell line B16-F1-R2 WT (30ug) (+)
Lane 2 Melanoma Cell line B16-F1-R2 KO (30ug) (+)