mAbs Against MOUSE Protein
P16
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | rat anti mouse |
| CLONE NAME: | PABLO33B |
| PROTEIN: | cyclin-dependent kinase inhibitor 2A |
| ANTIGEN USED: | HIS-GST-mp16 recombinant protein (full length protein) |
| FUSION PARTNER: | myeloma p3-NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG2a |
| SPECIES REACTIVITY: | mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
References
Therapeutic senescence via GPCR activation in synovial fibroblasts facilitates resolution of arthritis. Trinidad Montero-Melendez, Ai Nagano, Claude Chelala, Andrew Filer, Christopher D. Buckley and Mauro Perretti. Nature Communications volume 11, Article number: 745 (2020)
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | PABLO33B | Neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | PABLO33B | Neat | supernatant | Ventana | Fibrosarcoma tissue / | nuclear | |||
| Immunofluorescence | |||||||||
Enlarge image
- PABLO33B is able to detect mouse p16 (CDKN2A) protein in immunocytochemistry
- To confirm that PABLO33B mAb recognizes mouse p16 (CDKN2A) protein, immunocytochemistry on frozen cytospins preparations of HEK-mp16-V5 transfected cells was performed. Cytospin preparation of V5-tagged mouse p15 protein was used as a negative control. Anti-V5 was used as a positive control.
Enlarge image
- PABLO33B mAb can be used to detect mouse p16 (CDKN2A) protein in paraffin tissues
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | PABLO33B | undiluted | supernatant | / | 16kDa | 16kDa | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- PABLO33B mAb is able to detect mouse p16 (CDKN2A) protein by WB.
- LANES
Lane 1 HEKmp16 (20ug) (+)
Lane 2 HEKmp15 (20ug) (-)
Enlarge image
- PABLO33B mAb is able to detect mouse p16 (CDKN2A) protein by WB.
- LANES
Lane 1 NS1 mouse cell line (100ug)
Lane 2 PAP1 mouse papilloma tissue (100ug)
Lane 3 PAP3 mouse papilloma tissue (100ug)