mAbs Against MOUSE Protein
p53
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | rat anti mouse |
| CLONE NAME: | POE316A |
| PROTEIN: | Mus musculus transformation related protein 53 (Trp53) |
| ANTIGEN USED: | His-GST-mp53 recombinant protein |
| FUSION PARTNER: | myeloma p3-NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG2a |
| SPECIES REACTIVITY: | mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
p53 also known as cellular tumor antigen p53 or phosphoprotein p53 or tumor suppressor p53 is a protein that in humans is encoded by the TP53 gene. The p53 protein is crucial in multicellular organisms, where it regulates the cell cycle and, thus, functions as a tumor suppressor, preventing cancer. As such, p53 has been described as "the guardian of the genome" because of its role in conserving stability by preventing genome mutation.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | POE316A | 1:2 | supernatant | mouse intestine / | nuclear | ||||
| Immunofluorescence | |||||||||
Enlarge image
- POE316A mAb in transfected cells
- Nuclear and cytoplasmic staining of POE316A mAb in transfected HEK293-mp53-GFP cells. Anti-GFP was used as positive control.
Enlarge image
- POE316A mAb in mouse paraffin tissues
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | POE316A | neat supernatant | supernatant | / | 43kDa | Available | ||
| Immunoprecipitation | ||||||||
Enlarge image
- Western Blotting of mp53 (POE316A)
- Western Blotting of mp53 using different mouse cell lines and HEK293T transfected cells.
Lane 1 Hek-mp53-GFP transfected cells (20ug) (+)
Lane 2 Hek-FGFR1-GFP transfected cells (20ug) (-)
Lane 3 Mefs mp53 ER/ER cells (100ug) (+)
Lane 4 Mefs mp53 ER/ER Ras GFP (100ug) (+)
Enlarge image
- Western Blotting of mp53 (POE316A)
- Predicted molecular weight: 43kDa
Observed molecular weight: 43kDa
Lanes
Lane 1 KSS mouse 1 lox/lox (30ug) (-)
Lane 2 KSS mouse 4 lox/lox (30ug) (-)
Lane 3 KSS mouse 6 p53 +/+ (30ug) (+)
Lane 4 KSS mouse 7 lox/lox (30ug) (-)
Lane 5 KNR1 mouse+CRE (30ug) (+)
Lane 6 KNR1 mouse+CRE+shp53 (30ug) (+/-)
Image kindly provided by Barbara Oldrini.