mAbs Against MOUSE Protein
PLK1
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | Rat monoclonal |
| CLONE NAME: | POE125A |
| PROTEIN: | Serine/threonine-protein kinase PLK1 |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/Q07832 |
| ANTIGEN USED: | HIS-hPlk1 full-length protein |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | Unknown |
| SPECIES REACTIVITY: | human and mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains.
References
Wachowicz P, Fernández-Miranda G, Marugán C, Escobar B, de Cárcer G. Genetic depletion of Polo-like kinase 1 leads to embryonic lethality due to mitotic aberrancies. Bioessays. 2016 Jul;38 Suppl 1:S96-S106. doi: 10.1002/bies.201670908. PubMed PMID: 27417127.
Trakala M, Partida D, Salazar-Roa M, Maroto M, Wachowicz P, de Cárcer G, Malumbres M. Activation of the endomitotic spindle assembly checkpoint and thrombocytopenia in Plk1-deficient mice. Blood. 2015 Oct 1;126(14):1707-14. doi: 10.1182/blood-2015-03-634402. PubMed PMID: 26185128.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | POE125A | Neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | POE125A | Neat | supernatant | Ventana | / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- POE125A is able to detect human and mouse Plk1 protein in immunocytochemistry
- To confirm that POE125A mAb recognizes human and mouse PLK1 protein, immunocytochemistry on frozen cytospin preparations of GFP-tagged hPLK1 and mPLK1 expressed in HEK293T cell line was performed.
Enlarge image
- POE125A mAb can be used to detect PLK1 protein in mouse paraffin tissues
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | POE125A | Neat | supernatant | / | 67kDa | 67kDa | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- POE125A mAb is able to detect mouse and human Plk1 protein by WB.
- LANES
Lane 1 Mefs-hPlk1-GFP (100ug) (+)
Lane 2 Mefs (100ug) (+)
mPlk1 endogenous expression in both lanes