mAbs Against HUMAN Protein
AID
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse anti human |
| CLONE NAME: | JUA51E |
| PROTEIN: | Activation induced cytidine deaminase |
| PROTEIN WEB: | http://www.hprd.org/summary?hprd_id=05585&isoform_id=05585_1&isoform_name=Isoform_1 |
| ANTIGEN USED: | EVDDLRDAFRMLGF peptide |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
Maturation of the antibody repertoire is mediated by two different mechanisms: class-switch recombination (CSR) and somatic hypermutation (SHM). These two processes are T cell dependent and occur in the germinal centres of secondary lymphoid organs. CSR leads to the production of antibodies of different isotypes whereas SHM leads to the selection of B cells expressing a BCR with high affinity for antigen. The activation-induced cytidine deaminase (AID) was recently shown to play a key role in these two mechanisms, demonstrating for the first time that these maturation processes share a common mechanism. There is evidence that AID is involved in the somatic DNA alterations required for CSR and SHM.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | JUA51E | 1:2 | Supernatant | Tris-EDTA | Novolink | Tonsil / | nuclear and cytoplasmic | ||
| Immunofluorescence | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | JUA51E | undiluted | supernatant | Tris-EDTA | Tonsil / | nuclear and cytoplasmic | Available | ||
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- JUA51E mAb in transfected cells.
- Cytoplasmic staining on frozen cytospin preparations of transfected HEK293T/AID cells using antibody JUA51E. HEK293T/p27 transfected cells were used as negative control. Anti-HA and anti-AID (EK2 clone) were used as positive controls.
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- JUA51E mAb in paraffin sections.
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- AID (JUA51E) immunofluorecence in human tonsil
- Anti-AID (EK2) was used as positive control.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | JUA51E | Undiluted | Supernatant | Daudi cell line / | 24kDa | 26kDa | Available | |
| Immunoprecipitation | ||||||||
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- Western Blotting of AID (JUA51E) using different human cell lines.
- Lane 1 Hek-AID-HA (30ug) (+)
Lane 2 Hek-AID-HA (30ug) (+)
Lane 3 Hek-PSF1 (30ug) (-)
Lane 4 Ramos cell line (100ug) (-)
Lane 5 Jurkat cell line (100ug) (-)
Lane 6 Daudi cell line (100ug) (+)
Lane 7 BL2 cell line (100ug) (-)
Lane 8 Alewife cell line (100ug) (-)
Lane 9 Human tonsil (100ug) (-)