mAbs Against HUMAN Protein
CD147 Basigin
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse monoclonal |
| CLONE NAME: | NK99C |
| PROTEIN: | Basigin/BSG |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/P35613 |
| ANTIGEN USED: | YT cell line |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
References
Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3, SLC16A8, SLC16A11 and SLC16A12 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | NK99C | Neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | NK99C | 1:80 | supernatant | / | Available | ||||
| Immunofluorescence | |||||||||
Enlarge image
- NK99C mAb in HEK transfected cells
- To confirm that NK99C mAb recognizes human CD147 protein, immunocytochemistry on frozen cytospin preparations of human CD147 expressed in HEK293 was performed. Anti-FLAG antibody was used as positive control. Cytospin preparation of human CPEB3 protein was used as a negative control.
Enlarge image
- NK99C antibody can be used to detect CD147 protein in human paraffin tissues.
- TISSUE SAMPLE Human tonsil
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | NK99C | 1:200 | Purified | Tonsil / | 42kDa | Available | ||
| Immunoprecipitation | ||||||||
Enlarge image
- NK99C mAb is able to detect human CD147 protein by WB.
- LANES
Lane 1 HEK-CD147 (10ug) (+)
Lane 2 HEK-CD63 (10ug) (-)
Lane 3 NK9 cell line (100ug) (+)
Lane 4 YT cell line (100ug) (+)
Lane 5 Peripheral Blood (100ug) (-)
Lane 6 Human Tonsil (100ug) (-)