mAbs Against HUMAN Protein
CD43
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse monoclonal |
| CLONE NAME: | 93F |
| PROTEIN: | Leukosialin |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/P16150 |
| ANTIGEN USED: | 697 cell line |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4+ T-cells and to a lesser extent by CD8+ T-cells.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 93F | Neat | supernatant | Tonsil / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 93F | 1:400 | supernatant | / | Available | ||||
| Immunofluorescence | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 93F | 1:50 | supernatant | Tonsil / | Available | ||||
Enlarge image
- 93F antibody can be used to detect CD43 protein in human frozen tissues.
- Tissue sample: human tonsil
Enlarge image
- 93F antibody can be used to detect CD43 protein in human paraffin tissues
Enlarge image
- 93F antibody can be used to detect CD43 protein in immunofluorescence
- TISSUE SAMPLE: Hodgkin’s Lymphoma
CD43 RED/PSGL1 GREEN/DAPI BLUE.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 93F | 1:2 | supernatant | Tonsil / | 115-135kDa | 115kDa | Available | |
| Immunoprecipitation | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 93F | 1:2 | supernatant | Tonsil / | Available | |||
Enlarge image
- 93F mAb is able to detect human CD43 protein by WB.
- LANES
Lane 1 Jurkat cell line (200ug) (+)
Lane 2 Karpas299 (200ug) (+)
Lane 3 Thymus (200ug) (+)
Lane 4 Raji cell line (200ug) (+)
Lane 5 Hut 78 cell line (200ug) (+)
Lane 6 Tonsil (200ug) (+)
Lane 7 Testicle (200ug) (-)
Lane 8 Daudi cell line (200ug) (-)
Lane 9 Colo205 cell line (200ug) (-)
Enlarge image
- Antibody 93F can be used to immunoprecipitate CD43 protein
- A. Immunoprecipitation of protein extracts from human tonsil, Hut78 cell line, human testicle and Daudi cell line with 93F (40ul/lane as supernatant) followed by western blotting with the same antibody.
B. Immunoprecipitation of protein extracts from human tonsil, Hut78 cell line, human testicle and Daudi cell line with 93F (40ul/lane used as supernatant) followed by western blotting with Dako anti-CD43 antibody.
C. Immunoprecipitation of protein extracts from human tonsil, Hut78 cell line, human testicle and Daudi cell line with Dako antibody (4ul/lane) followed by western blotting with 93F antibody.
D. Immunoprecipitation of protein extracts from human tonsil, Hut78 cell line, human testicle and Daudi cell line with Dako antibody (4ul/lane) followed by western blotting with the same antibody.