mAbs Against HUMAN Protein
CD63
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse monoclonal |
| CLONE NAME: | KILL150A |
| PROTEIN: | CD63 antigen |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/P08962 |
| ANTIGEN USED: | YT and NK92 cell lines |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | KILL150A | Neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | KILL150A | 1:30 | supernatant | / | Available | ||||
| Immunofluorescence | |||||||||
Enlarge image
- KILL150A mAb is able to detect human CD63 protein in immunocytochemistry.
- To confirm that KILL150A mAb recognizes human CD63 protein, immunocytochemistry on frozen cytospins preparations of human CD63 expressed in HEK293 was performed. Anti GFP mAb was used as positive control. Cytospin preparation of human ECT2 protein was used as a negative control.
Enlarge image
- KILL150A antibody can be used to detect CD63 protein in human paraffin tissues.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | KILL150A | Neat | supernatant | YT cell line / | 40-60kDa | 30-60kDa due to glicosilation | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- KILL150A mAb is able to detect human CD63 protein by WB
- LANES
Lane 1 Hek-CD63-GFP (10ug) (+)
Lane 2 Hek-CD147 (10ug) (-)
Lane 3 RAJI cell line (100ug) (+)
Lane 4 RAMOS cell line (100ug) (+)
Lane 5 YT cell line (100ug) (+)
Lane 6 NK92 cell line (100ug) (+)
Lane 7 MOLT4 cell line (100ug) (-)
Lane 8 JURKAT cell line (100ug) (+)
Lane 9 HH cell line (100ug) (+)
| FLOW CYTOMETRY | Clone | Dilution | +/- control | Type of fluorocrom | Protocol |
|---|---|---|---|---|---|
Recommended Result obtained is satisfactory. The reagent can be use in this application | KILL150A | 1:400 purified antibody | YT cell line / |
Enlarge image
- KILL150A antibody can be used to detect CD63 protein in flow cytometry technique.