mAbs Against HUMAN Protein
CPEB4
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse anti-human |
| CLONE NAME: | ERE93C |
| PROTEIN: | mouse CPEB4 (Cytoplasmic polyadenylation element-binding protein 4) |
| PROTEIN WEB: | http://www.uniprot.org/uniprot/Q17RY0 |
| ANTIGEN USED: | HIS-GST-mCPEB4 (1-375aa) recombinant protein |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | human and mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
References
Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers. Pérez-Guijarro E, Karras P, Cifdaloz M, Martínez-Herranz R, Cañón E, Graña O, Horcajada-Reales C, Alonso-Curbelo D, Calvo TG, Gómez-López G, Bellora N, Riveiro-Falkenbach E, Ortiz-Romero PL, Rodríguez-Peralto JL, Maestre L, Roncador G, de Agustín Asensio JC, Goding CR, Eyras E, Megías D, Méndez R, Soengas MS.
Nat Commun. 2016 Nov 18; 7:13418.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | ERE93C | neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | ERE93C | 1:1500 | purified | / | Available | ||||
| Immunofluorescence | |||||||||
Enlarge image
- ERE93CmAb in HEK293T transfected cells.
- Anti-Flag was used as positive control. HEK-TOX-Flag transfected cells were used as negative control.
Enlarge image
- ERE93C staining in human paraffin sections.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | ERE93C | melanoma tissue / | Available | |||||
| Immunoprecipitation | ||||||||
Enlarge image
- Western Blotting of CPEB4 (ERE93C) using different human cell lines.
- Predicted molecular weight: 80kDa
Observed molecular weight: 80kDa
Lanes
Lane 1 Foreskin melanocytes (prep 1)
Lane 2 Foreskin melanocytes (prep 2)
Lane 3 Melanoma cell line SK-Mel-5
Lane 4 Melanoma cell line SK-Mel-28
Lane 5 Melanoma cell line SK-Mel-29
Lane 6 Melanoma cell line SK-Mel-103
Lane 7 Melanoma cell line UACC-62
Lane 8 RWP-1 cell line (pancreatic cancer)
Lane 9 HeLa cell line (cervical cancer)
Lane 10 U251 cell line (glioma)
Lane 11 Melanoma cell line SK-Mel-28 transduced with pLK.O1_shControl
Lane 12 Melanoma cell line SK-Mel-28 transduced with pLK.O1_shCPEB4
Lane 13 Melanoma cell line SK-Mel-28 transduced with pLV empty vector
Lane 14 Melanoma cell line SK-Mel-28 transduced with pLV-CPEB4
Image provided by Eva Pérez.