mAbs Against HUMAN Protein
Estrogen Receptor Alpha
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse anti human |
| CLONE NAME: | CRET94D |
| PROTEIN: | Estrogen Receptor Alpha (ESR1) |
| PROTEIN WEB: | http://www.hprd.org/alternate?hprd_id=00589&isoform_id=00589_4&isoform_name=Isoform_1 |
| ANTIGEN USED: | MBP-CRE-ERT recombinant protein |
| FUSION PARTNER: | myeloma p3-NS1/Ag4-1 (NS1) cell |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human and mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
The oestrogen receptor alpha (ESR1) is a transcription factor that potentiates the response to diverse stimuli, including oestrogen and growth factors, in various tissue types. Its recruitment to the DNA is directly regulated by the chromatin landscape, inclusive of chromatin compaction and epigenetic modifications.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | CRET94D | 1:40 | supernatant | / | Available | ||||
| Immunofluorescence | |||||||||
Enlarge image
- CRET94D in transfected cells
- To confirm that CRET94D mAb recognized human Estrogen receptor protein, immunohistochemistry on frozen cytospins preparations of CRE-ERT expressed in Hek293T was performed. Cytospin preparation of E4F1 transfected cells was used as negative control.
Enlarge image
- CRET94D in human breast cancer
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | CRET94D | 1:5 | supernatant | / | 66kDa | mouse | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- CRET94D in Western blotting
- Lane 1 Hek-CRE-ERT (20ug) (+)
Lane 2 HBL100 cell line (100ug) (-)
Lane 3 MCF7 cell line (100ug) (+)
Lane 4 Human tonsil (100ug) (-)
Lane 5 ZR75-1 cell line (human breast carcinoma) (100ug)(+)