mAbs Against HUMAN Protein
GASDERMIN-1
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse anti human |
| CLONE NAME: | GAS120C |
| PROTEIN: | Gasdermin-A (GSDMA) |
| PROTEIN WEB: | http://www.uniprot.org/uniprot/Q96QA5 |
| ANTIGEN USED: | His-Gasdermin1 recombinant C-term protein (208-403aa) |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG2b |
| SPECIES REACTIVITY: | human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | GAS120C | neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | GAS120C | 1:5 | supernatant | Tris-EDTA | Human breast cancer / | ||||
| Immunofluorescence | |||||||||
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- GAS120C mAb in transfected cells
- To confirm that GAS120C mAb recognized human Gasdemin1 protein, immunohistochemistry on frozen cytospins preparations of Gasdermin1 isoforms 1 and 2 expressed in Hek293T was performed. Anti-HA antibody was used as positive control. Hek-Hes1-HA transfected cells were used as negative control.
Enlarge image
- GAS120C antibody can be used to detect human Gasdermin1 protein in paraffin embedded tissue samples.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | GAS120C | neat | supernatant | Colo205 cell line / HeLa cell line | 49kDa | 49kDa | Available | |
| Immunoprecipitation | ||||||||
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- GAS120C mAb is able to detect Gasdermin1 protein by WB.
- Lane 1 Gasdermin-His recombinant protein (20ug) (+)
Lane 2 MCF7 cell line (100ug) (-)
Lane 3 HBL100 cell line (100ug) (-)
Lane 4 Hela cell line (100ug) (-)
Lane 5 Colo205 cell line (100ug)(+)
Lane 6 BL2 cell line (100ug) (-)
Lane 7 Jurkat cell line (100ug) (-)