mAbs Against HUMAN Protein
Leu1
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | CD5/54/F6 |
| PROTEIN: | Leu1 (CD5) |
| PROTEIN WEB: | https://www.omim.org/entry/153340 |
| ANTIGEN USED: | Peptide from the intracellular region of CD5 (SSMQPDNSSDSDYDLHGAQRL) |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human, Bovine, Chicken, Horse, Guinea Pig, Opossum, Pig, Primate, Rat and Rabbit |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | IHC WB |
Description
CD5 is a surface glycoprotein present on peripheral T cells (>95%) and B-cell chronic lymphocytic leukaemia cells that binds CD72. CD5 has utility for the monitoring of T-cell numbers in peripheral blood and the identification of leukaemias of T cell origin. Absence of the CD5 marker on childhood ALL cells is associated with a more favourable prognosis.
Publication describing antibody characterization/validation
J Immunol. 1993 Jun 15;150(12):5429-35.
Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies.
Jones M1, Cordell JL, Beyers AD, Tse AG, Mason DY.
PMID: 8515069
https://www.ncbi.nlm.nih.gov/pubmed/8515069
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| CD5/54/F6 | neat | supernatant | Tris EDTA | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- CD5/54/F6 Tonsil
- Tonsil stained with neat supernatant from CD5/54/F6 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
- CD5/54/F6 Tonsil
- Tonsil stained with neat supernatant from CD5/54/F6 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
- CD5/54/F6
- Tonsil stained with neat supernatant from bcl-2/100 hybridoma, detection using Dako Envision kit, 10x power image