mAbs Against HUMAN Protein
LEU2
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | C8/144B |
| PROTEIN: | Leu2 (CD8) |
| PROTEIN WEB: | https://www.omim.org/entry/186910 |
| ANTIGEN USED: | Synthetic peptide (the 13 C-terminal residues of the cytoplasmic domain of human CD8a) |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | IHC IF IP WB |
Description
CD8 is a T cell co-receptor that recognises, together with the T cell receptor, MHC class I molecules. CD8 is present on human suppressor/cytotoxic T cells which make up 30% of circulating T cells. C8/144B can be used to detect these cells in tissue sections and also their neoplastic counterparts.
Publication describing antibody characterization/validation
J Clin Pathol. 1992 Dec;45(12):1084-8.
Immunohistological detection of human cytotoxic/suppressor T cells using antibodies to a CD8 peptide sequence.
Mason DY1, Cordell JL, Gaulard P, Tse AG, Brown MH.
PMID: 1479035
https://www.ncbi.nlm.nih.gov/pubmed/1479035
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| C8/144B | neat | supernatant | Tris EDTA | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- C8/144B Tonsil
- Tonsil stained with neat supernatant from bcl-2/100 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
- C8/144B Tonsil
- Tonsil stained with neat supernatant from bcl-2/100 hybridoma, detection using Dako Envision kit, 20x power image