mAbs Against HUMAN Protein
MAP17 - PDZK1
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse monoclonal |
| CLONE NAME: | 165C |
| PROTEIN: | PDZK1-interacting protein 1-Protein DD96 |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/Q13113#names_and_taxonomy |
| ANTIGEN USED: | GST-MAP17 |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Millipore |
Description
May play an important role in tumor biology.
References
MAP17 and SGLT1 protein expression levels as prognostic markers for cervical tumor patient survival. Perez M, Praena-Fernandez JM, Felipe-Abrio B, Lopez-Garcia MA, Lucena-Cacace A, Garcia A, Lleonart M, Roncador G, Marin JJ, Carnero A. PLoS One. 2013;8 (2).
MAP17 and the double-edged sword of ROS. Carnero A. Biochim Biophys Acta. 2012 Aug;1826 (1):44-52.
p38α limits the contribution of MAP17 to cancer progression in breast tumors. Guijarro MV, Vergel M, Marin JJ, Muñoz-Galván S, Ferrer I, Ramon y Cajal S, Roncador G, Blanco-Aparicio C, Carnero A. Oncogene. 2012 Oct 11;31(41):4447-59.
MAP17 inhibits Myc-induced apoptosis through PI3K/AKT pathway activation. Guijarro MV, Link W, Rosado A, Leal JF, Carnero A. Carcinogenesis. 2007 Dec;28(12):2443-50.
MAP17 enhances the malignant behavior of tumor cells through ROS increase. Guijarro MV, Leal JF, Blanco-Aparicio C, Alonso S, Fominaya J, Lleonart M, Castellvi J, Ramon y Cajal S, Carnero A. Carcinogenesis. 2007 Oct;28(10):2096-104.
MAP17 overexpression is a common characteristic of carcinomas. Guijarro MV, Leal JF, Fominaya J, Blanco-Aparicio C, Alonso S, Lleonart M, Castellvi J, Ruiz L, Ramon Y Cajal S, Carnero A. Carcinogenesis. 2007 Aug;28(8):1646-52.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 165C | Neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 165C | 1:5 | supernatant | Kidney / | Available | ||||
| Immunofluorescence | |||||||||
Enlarge image
- 165C is able to detect human MAP17 protein in immunocytochemistry
- To confirm that 165C mAb recognizes human MAP17 protein, immunocytochemistry on frozen cytospin preparations of GFP-tagged MAP17 expressed in HEK293T was performed. Cytospin preparation of GFP transfected cells was used as negative control.
Enlarge image
- 165C mAb can be used to detect MAP17 protein in human paraffin tissues.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 165C | Neat | supernatant | Kidney / | 12kDa | 12kDa | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- 165C mAb is able to detect human MAP17 protein by WB.
- LANES
Lane 1 Human kidney (100ug) (+)
Lane 2 GST-MAP17 recombinant protein (0,1ug) (+)
Lane 3 GST-MAP17 recombinant protein (0,2ug) (+)