mAbs Against HUMAN Protein
MASTL
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse anti human |
| CLONE NAME: | RIPLY74C |
| PROTEIN: | Microtubule associated serine/threonine kinase-like |
| PROTEIN WEB: | http://www.ncbi.nlm.nih.gov/protein/AAH09107.1 |
| ANTIGEN USED: | GST-hMASTL recombinant full length protein |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human and mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
MASTL (microtubule associated serine/threonine kinase-like), also known as GW, THC2 or serine/threonine-protein kinase greatwall, is an 879 amino acid serine/threonine kinase regulates mitosis entry and maintenance. Depletion of MASTL delays the G2-to-M phase transition, is linked to a prolonged mitosis and sensitizes cells to mitotic death.
Publication describing antibody characterization/validation
Álvarez-Fernández M, Sanz-Flores M, Sanz-Castillo B, Salazar-Roa M, Partida D, Zapatero-Solana E, Ali HR, Manchado E, Lowe S,VanArsdale T, Shields D, Caldas C, Quintela-Fandino M, Malumbres M. Therapeutic relevance of the PP2A-B55 inhibitory kinase MASTL/Greatwall in breast cancer. Cell Death Differ. 2018 May;25(5):828-840.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | RIPLY74C | 1:15 | supernatant | Tonsil / | mouse | Available | |||
| Immunofluorescence | |||||||||
Enlarge image
- RIPLY74C mAb in transfected cells
- Cytospin preparations of transfected HEK293T/v5-hMASTL and HEK293T/V5-mMASTL cells using antibody RIPLY74C. Anti-V5 antibody was used as positive control.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | RIPLY74C | 1:50 | neat supernatant | Raji cell line / | 97kDa | 120kDa | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- Western Blotting of MAST (RIPLY74C) using different human cell lines.
- Lane 1 Hek-hMASTL transfected cells (20ug) (+)
Lane 2 Hek-mMASTL transfected cells (20ug) (+)
Lane 3 Hek-FLT4 transfected cells (20ug) (-)
Lane 4 Raji cell line (100ug)(+)
Lane 5 Ramos cell line (100ug)(+)
Lane 6 Dana cell line (100ug)(+)
Lane 7 MT-2 cell line (100ug)(+)
Lane 8 human tonsil (100ug)(-)
Lane 9 Human testis (100ug)(-)
Enlarge image
- Western Blotting of MASTL (RIPLY74C) using different cell lines
- Lane 1 293T cell mock transfected (40ug)
Lane 2 293T cells V5-mMASTL (40ug)
Lane 3 HeLa cells shCtrl transfected (40ug)
Lane 4 HeLa cells shMASTL transfected (40ug)