mAbs Against HUMAN Protein
Neutrophil elastase
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | NP57 |
| PROTEIN: | Neutrophil elastase |
| PROTEIN WEB: | https://www.omim.org/entry/130130 |
| ANTIGEN USED: | Neutrophil granule proteins |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG1 kappa |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | IHC IF IP WB |
Description
Human Neutrophil Elastase (HNE) belongs to the chymotrypsin family of serine proteases able to solubilize fibrous elastin, an important extracellular matrix protein that has the unique property of elastic recoil, and plays a major structural function in lungs, arteries, skin and ligaments. HNE is primarily located in the azurophil granules of polymorphonuclear leukocytes, but has also been detected in nuclear membrane, Golgi complex, ER and the mitochondria of these cells. The enzyme is involved in the tissue destruction and inflammation and implicated in numerous diseases, including emphysema, chronic obstructive pulmonary disease, cystic fibrosis, ischemic-reperfusion injury and rheumatoid arthritis.
NP57 is useful for the differential diagnosis of acute leukaemia by APAAP labeling of cell smears. It may also be of value for the histopathological diagnosis of tumour deposits in myeloid leukaemia and for the detection of neutrophils in paraffin sections
Publication describing antibody characterization/validation
J Clin Pathol. 1988 Aug;41(8):853-60.
Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells.
Pulford KA1, Erber WN, Crick JA, Olsson I, Micklem KJ, Gatter KC, Mason DY.
PMID: 2844860
https://www.ncbi.nlm.nih.gov/pubmed/2844860
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| NP57 | neat | supernatant | Citrate | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- NP57 Tonsil
- Tonsil stained with neat supernatant from NP57 hybridoma, detection using Dako Envision kit, 40x power image