mAbs Against HUMAN Protein
NSE2
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse monoclonal |
| CLONE NAME: | 215C |
| PROTEIN: | E3 SUMO-protein ligase NSE2 |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/Q96MF7 |
| ANTIGEN USED: | NSE2-GST |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Acts as an E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, and maybe the cohesin components RAD21 and STAG2.
References
NSMCE2 suppresses cancer and aging in mice independently of its SUMO ligase activity. Jacome A, Gutierrez-Martínez P, Schiavoni F, Tenaglia E, Martinez P, Rodríguez-Acebes S, Lecona E, Murga M, Méndez J, Blasco MA, Fernandez-Capetillo O. EMBO J. 2015 Nov 3;34(21):2604-19.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 215C | Neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 215C | 1:20 | Purified | Tris-EDTA | Novolink | Tonsil / | nuclear | Available | |
| Immunofluorescence | |||||||||
Enlarge image
- 215C is able to detect human NSE2 protein in immunocytochemistry
- To confirm that 215C mAb recognizes human NSE2 protein, immunocytochemistry on frozen cytospin preparations of GFP-tagged NSE2 expressed in HEK293T was performed. Cytospin preparation of GFP transfected cells was used as negative control.
Enlarge image
- 215C mAb can be used to detect NSE2 protein in human paraffin tissues
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 215C | Neat | supernatant | Ovary / | 28kDa | >30kDa | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- 215C mAb is able to detect human NSE2 protein by WB.
- LANES
Lane 1 NSE2-GST (0.1ug) (+)
Lane 2 Tonsil (100ug) (-)
Lane 3 Testicle (100ug) (-)
Lane 4 Ovary (100ug) (+)