mAbs Against HUMAN Protein
Nucleophosmin
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | NA24 |
| PROTEIN: | Nucleophosmin |
| PROTEIN WEB: | https://www.omim.org/entry/164040 |
| ANTIGEN USED: | Nucleophosmin recombinant protein |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | ChIP IHC IF IP WB |
Description
Nucleophosmin (NPM) also called B23, nutramin and NO38 is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity and centrosome duplication. It is also a crucial regulator of p53; it is involved in the acute response of mammalian cells to environmental stress, such as UV rays. NPM continuously shuttles between the nucleus and cytoplasm. NPM also localises between the paired centrioles of the centrosome and dissociates upon the phosphorylation of on Thr199 by CDK2/cyclin E prior to the initiation of centrosome duplication, an essential process for successul chromosome segregation during mitosis. The down-regulation of this protein results in the abnormal amplification of centrosomes which suggests that this protein may act as a a suppressor of centrosome duplication.
Publication describing antibody characterization/validation
Blood. 1999 Jan 15;93(2):632-42.
Detection of normal and chimeric nucleophosmin in human cells.
Cordell JL1, Pulford KA, Bigerna B, Roncador G, Banham A, Colombo E, Pelicci PG, Mason DY, Falini B.
PMID: 9885226
https://www.ncbi.nlm.nih.gov/pubmed/9885226
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| NA24 | neat | supernatant | Tris EDTA | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- NA24 Tonsil
- Tonsil stained with neat supernatant from NA24 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
- NA24 Tonsil
- Tonsil stained with neat supernatant from bcl-2/124 hybridoma, detection using Dako Envision kit, 20x power image