mAbs Against HUMAN Protein
p15/p16
| STATUS: |  |
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| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
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| STATUS: | Validated |
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| TYPE: | rat monoclonal |
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| CLONE NAME: | PABLO228C |
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| PROTEIN: | CDKN2a and CDKN2b |
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| ANTIGEN USED: | HIS-GST-mp16 recombinant protein |
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| FUSION PARTNER: | myeloma p3-NS1/Ag4-1 (NS1) cells |
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| ISOTYPE: | IgG1 |
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| SPECIES REACTIVITY: | human and mouse |
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| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
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Applications
IHC Techniques
| IHC Techniques |
Clone |
Dilution |
Antibody concentration |
Antigen retrieval method |
Visualization kit |
+/- control |
Protein localization |
Positivity in other species |
Protocol |
| Frozen tissue and cytospins |
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| Paraffin tissue |
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Recommended Result obtained is satisfactory. The reagent can be use in this application | PABLO228C |
1:5 |
supernatant |
|
|
/ |
|
|
Available |
| Immunofluorescence |
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Enlarge image
- PABLO228c mAb can be used to detect p15/p16 proteins in human paraffin tissues
