mAbs Against HUMAN Protein
PLK5
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse monoclonal |
| CLONE NAME: | MUSE286C |
| PROTEIN: | Inactive serine/threonine-protein kinase PLK5 |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/Q496M5 |
| ANTIGEN USED: | HIS-GST-hPLK5 full length protein |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
Inactive serine/threonine-protein kinase that plays a role in cell cycle progression and neuronal differentiation.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | MUSE286C | Neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | MUSE286C | Neat | supernatant | / | Available | ||||
| Immunofluorescence | |||||||||
Enlarge image
- MUSE286C mAb is able to detect human PLK5 protein in immunocytochemistry
- To confirm that MUSE286C mAb recognizes human PLK5 protein, immunocytochemistry on frozen cytospin preparations of GFP-tagged human PLK5 expressed in HEK293 was performed. Cytospin preparation of human GFP-tagged human PLK1 protein was used as a negative control. Labeling with the anti-GFP antibody confirmed transfection efficiency.
Enlarge image
- MUSE286C mAb can be used to detect PLK5 protein in human paraffin tissues
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | MUSE286C | Neat | supernatant | / | 36kDa | >37kDa | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- MUSE286C mAb is able to detect mouse and human PLK5 protein by WB.
- LANES
Lane 1 HEK-PLK5-GFP (15ug) (+)
Lane 2 HEK-PLK1 (15ug) (-)
Lane 3 Human brain (100ug) (+)
Lane 4 Human tonsil (100ug) (-)
Lane 5 U87MG cell line (100ug) (+)
Lane 6 U251 cell line (100ug) (+)
Lane 7 KELLY cell line (100ug) (+)
Lane 8 LAN1 cell line (100ug) (+)
Lane 9 SH-SY5Y cell line (100ug) (+)