mAbs Against HUMAN Protein
RTN4
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse monoclonal |
| CLONE NAME: | LIG198B |
| PROTEIN: | Reticulon-4 |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/Q9NQC3 |
| ANTIGEN USED: | HDLM2 cell line |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG3 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
Required to induce the formation and stabilization of endoplasmic reticulum (ER) tubules. They regulate membrane morphogenesis in the ER by promoting tubular ER production. They influence nuclear envelope expansion, nuclear pore complex formation and proper localization of inner nuclear membrane proteins. However each isoform have specific functions mainly depending on their tissue expression specificities.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | LIG198B | Neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | LIG198B | 1:50 | supernatant | / | Available | ||||
| Immunofluorescence | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | LIG198B | Neat | supernatant | / | Available | ||||
Enlarge image
- LIG198B is able to detect human RTN4 protein in immunocytochemistry
- To confirm that LIG198B mAb recognizes human RTN4 protein, immunocytochemistry on frozen cytospin preparations of V5-tagged RTN4 isoform2 expressed in HEK293T was performed. Anti-V5 was used as a positive control.
Enlarge image
- LIG198B mAb can be used to detect RTN4 protein in human paraffin tissues
Enlarge image
- LIG198B mAb can be used to detect RTN4 protein by immunofluorescence
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | LIG198B | Neat | supernatant | / | Available | |||
| Immunoprecipitation | ||||||||
Enlarge image
- LIG198B mAb is able to detect human RTN4 protein by WB.
-
Isoform 1: 130KDA
Isoform 2: 40KDA
Isoform 3: 22KDA
Isoform 4: 106KDA
Isoform 5: 42KDA
Isoform 6: 108KDA
Observed molecular weight: 140-160Kda and 48-60kda
The samples were divided in to two gels to allow the observation of all the isoforms of the RTN4 protein.
LANES
Lane 1 Hek-RTN4 isoform2 (50ug)
Lane 2 Hek-CD10 (50ug)
Lane 3 U87MG (100ul)
Lane 4 SBN75 (100ul)
Lane 5 Testicle (100ul)
Lane 6 Brain (100ul)
Lane 7 U251 (100ul)
Lane 8 SKML19 (100ul)
Lane 9 Daudi (100ul)