mAbs Against HUMAN Protein
SA1 - STAG1
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | Rat monoclonal |
| CLONE NAME: | SUSI63B |
| PROTEIN: | Cohesin subunit SA-1 |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/Q8WVM7 |
| ANTIGEN USED: | mSA-1-MBP recombinant protein (N terminal fragment 225 aa; 99% identity with human SA-1) |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG2a |
| SPECIES REACTIVITY: | human and mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | SUSI63B | Neat | supernatant | Tonsil / | Available | ||||
| Immunofluorescence | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | SUSI63B | Neat | supernatant | / | |||||
Enlarge image
- SUSI63B mAb can be used to detect SA-1 protein in human paraffin tissues
Enlarge image
- SUSI63B mAb can be used to detect SA-1 protein by immunofluorescence in mouse and human cells
- HeLa cells fixed with paraformaldehyde and permeabilized with 0.2% Triton X-100
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | SUSI63B | Neat | supernatant | Hela cell line / | 144kDa | 144kDa | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- SUSI63B mAb is able to detect endogenous mouse and human SA-1 protein by WB.
- LANES
Lane 1 Human spleen (100ug) (+)
Lane 2 Human testicle (100ug) (+)
Lane 3 Jurkat cell line (100ug) (+)
Lane 4 Colo205 cell line (100ug) (+)
Lane 5 HeLa cell line (100ug) (+)
Lane 6 Molt-4 cell line (100ug) (+)
Lane 7 MCF-7 cell line (100ug) (+)
Lane 8 RAW cell line (100ug) (+)
Lane 9 HEK-mSA-1 transfected cells (10ug) (+)
| FLOW CYTOMETRY | Clone | Dilution | +/- control | Type of fluorocrom | Protocol |
|---|---|---|---|---|---|
Recommended Result obtained is satisfactory. The reagent can be use in this application | SUSI63B | / |
Enlarge image
- SUSI63B mAb can be used to detect human SA-1 protein by flow cytometry
- HeLa cells mock transfected or transfected with siRNA against hSA-1