mAbs Against HUMAN Protein
TdT
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse anti human |
| CLONE NAME: | 41A |
| PROTEIN: | DNA nucleotidylexotransferase |
| PROTEIN WEB: | http://www.ncbi.nlm.nih.gov/protein/26007035 |
| ANTIGEN USED: | HIS-TDT recombinant protein |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG2a |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Abcam and Biolegend |
Description
Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
References
Susan McClory, Tiffany Hughes, Aharon G Freud, Edward L Briercheck, Chelsea Martin, Anthony J Trimboli, Jianhua Yu, Xiaoli Zhang, Gustavo Leone, Gerard Nuovo, Michael A Caligiuri. Evidence for a stepwise program of extrathymic T cell development within the human tonsil. The Journal of Clinical Investigation. 2 April 2012.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 41C | Neat supernatant | / | ||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 41C | 1:10 | Supernatant | Thymus / | Available | ||||
| Immunofluorescence | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 41C | 1:50 | Supernatant | / | Available | ||||
Enlarge image
- 41C mAb in HEK293T-TdT transfected cells.
- To confirm that 41C mAb recognized human TdT protein, immunohistochemistry on frozen cytospins preparations of human TdT expressed in HEK293T was performed. Cytospin preparation of human BTLA protein was used as a negative control.
Enlarge image
- 41C mAb in human thymus tissue.
Enlarge image
- 41C mAb can be used to detect human TDT protein by IF.
- TdT (red) and DAPI (blue) in human thymus.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 41C | Neat supernatant | Thymus / | 58kDa | 58kDa | Available | ||
| Immunoprecipitation | ||||||||
Enlarge image
- 41C mAb is able to detect human TdT protein by WB
- Lane 1 Hek-TdT (30цg) (+)
Lane 2 Hek-Timp2 (30цg) (-)
Lane 3 Human thymus (100цg) (+)
Lane 4 Human testis (100цg) (-)