mAbs Against HUMAN Protein
VSTM1
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| CONTACT INFORMATION: | Center for Proteomics, University of Rijeka |
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| TYPE: | monoclonal |
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| CLONE NAME: | hVSTM1.07 |
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| PROTEIN: | VSTM1 |
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| ANTIGEN USED: | hVSTM1 ectodomain |
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| FUSION PARTNER: | hIgG1 Fc |
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| ISOTYPE: | IgG1 |
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| SPECIES REACTIVITY: | human |
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| PREPARATION AND STORAGE: | Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles. |
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| APP RECOMMENDED: | Flow cytometry |
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Description
V-set and transmembrane domain containing 1 (VSTM1) encodes two main splicing isoforms, VSTM1-v1 and VSTM1-v2 (Guo, Cell Immunol 2012, 278: 136–142; Steevels, J Immunol. 2010;184:4741–8). VSTM1-v1 is a type I membrane molecule and is mainly expressed in human peripheral blood granulocytes and monocytes (Steevels, J Immunol. 2010;184:4741–8). VSTM1-v2 is a soluble protein that can promote the differentiation and activation of Th17 cells (Guo, Cell Immunol 2012, 278: 136–142). In addition, VSTM1 expression has been associated with rheumatoid arthritis and tumors (Wang, 2016, PLoS ONE 11(1); Zhou, 2016, Apoptosis 21(10):1179-90). Our anti-hVSTM1 antibody hVSTM1.07 works in ELISA (on immunogen) and in FACS (on human cells).
Applications
FLOW CYTOMETRY
| FLOW CYTOMETRY |
Clone |
Dilution |
+/- control |
Type of fluorocrom |
Protocol |
| hVSTM1.07 |
1:100 |
/ isotype control antibody |
FITC |
Available |
Enlarge image
- Anti human VSTM1 antibody (clone VSTM1.07)
- Overlay histogram showing CD14+ hPBMCs stained with 1:100 VSTM1.07 Ab (red line) and FITC-conjugated anti-mouse IgG. Isotype control antibody (black line) was mouse IgG used under the same conditions.
