EuroMAbNet: European Monoclonal Antibodies Network

Articles about antibody validation

2024

• MAbs poorly performing, November 2014 (PDF 0.7 MB)

2020

• When antibodies mislead: the quest for validation. Nature Article, September 2020

2019

• Antibody validation for Western blot: By the user, for the user. JBC Article, November 2019 (PDF 1.1 MB)

2018

• Quality testing for antibodies is gaining traction. Nature Article, August 2018
• Antibody validation: A view from the mountains (PDF 450 KB)
• The antibody horror show: an introductory guide for the perplexed (PDF 180 KB)
• The path to VICTORy - a beginner’s guide to success using commercial research antibodies (PDF 310 KB)

2017

• The challenges with the validation of research antibodies. F1000Research, February 2017

2016

• A proposal for validation of antibodies. Nature Methods, September 2016
• The need for improved education and training in research antibody usage and validation practices. BioTechniques, Vol. 61, No. 1, July 2016, pp. 16-18
• Quality Issues of Research Antibodies. Anal Chem Insights. 2016; 11: 21-27
• Introducing the Affinity Binder Knockdown Iniciative. EuPA Open Proteomics Volume 10, March 2016, Pages 56-58

2015

• Reproducibility Standardize antibodies used in research. Nature. 2015 Feb 5;518(7537):27-9
• Reproducibility crisis: Blame it on the antibodies. Nature. 2015 May 21;521(7552):274-6.
• An open letter to our readers on the use of antibodies J Comp Neurol. 2005 Dec 26;493(4):477-8

2014

• When Tissue Antigens and Antibodies Get Along: Revisiting the Technical Aspects of Immunohistochemistry—The Red, Brown, and Blue Technique (PDF 2.5 MB)
• Controls for Immunohistochemistry: The Histochemical Society's Standards of Practice for Validation of Immunohistochemical Assays (PDF 280 KB)
• Principles of analytic validation of immunohistochemical assays: Guideline from the College of American Pathologists Pathology and Laboratory Quality Center. Arch Pathol Lab Med. 2014 Nov;138(11):1432-43
• Antibody validation of immunohistochemistry for biomarker discovery: recommendations of a consortium of academic and pharmaceutical based histopathology Methods. Nov;70(1):34-8. doi: 10.1016 researcher
• Commercial antibodies and their validation. Version 2. F1000Res. 2014 Oct 2 [revised 2014 Oct]
• Editorial: Antibody Can Get It Right: Confronting Problems of Antibody Specificity and Irreproducibility. Mol Endocrinol. 2014 Sep; 28(9): 1403-140

2013

• Reporting research antibody use: how to increase experimental reproducibility. Version 2. F1000Res. 2013; 2: 153

2012

• Specificity controls for immunocytochemistry: the antigen preadsorption test can lead to inaccurate assessment of antibody specificity. J Histochem Cytochem. 2012 Mar;60(3):174-87

2011

• Controls for Immunocytochemistry An Update. J Histochem Cytochem. 2011 Jan;59(1):6-12.

2010

• Antibody validation. Biotechniques. 2010 Mar;48(3):197-209

2009

• The Dark Side of the Immunohistochemical Moon Industry. J Histochem Cytochem. 2009 Dec;57(12):1099
• Commercial antibodies: the good, bad, and really ugly. J Histochem Cytochem. 2009 Jan;57(1):7-8

2008

• Suggested guidelines for immunohistochemical techniques in veterinary. J Vet Diagn Invest. 2008 Jul;20(4):393-41
• Specificity of immunoreaction the importance of Testing Specificity in Each Method. he Journal of Neuroscience, 10 September 2008, 28(37): 9083-9086
• Lack of specificity of commercially available antisera: better specifications needed. J Histochem Cytochem. 2008 Dec;56(12):1099-111
• Is my antibody-staining specific? How to deal with pitfalls of immunohistochemistry. Eur J Neurosci. 2008 Dec;28(12):2365-70

2006

• Antibodies as Valuable Neuroscience Research Tools versus Reagents of Mass Distraction. J Neurosci. 2006 Aug 2;26(31):8017-2

2005

• An Open Letter to Our Readers on the Use of Antibodies (PDF 50 KB)

2003

• Magic Peptides Magic Antibodies: Guidelines for Appropriate Controls for Immunohistochemistry (PDF 50 KB)