mAbs Against MOUSE Protein
CD38 (rat)
| STATUS: |  |
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| CONTACT INFORMATION: | Immunology Unit, Faculty of Medicine and Medical Sciences, University of Barcelona |
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| STATUS: | Validated |
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| TYPE: | mouse anti rat |
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| CLONE NAME: | CD38.14.27 |
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| PROTEIN: | - |
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| ANTIGEN USED: | Rat hepatic stellate cells (HSCs) |
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| FUSION PARTNER: | Ns1 |
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| ISOTYPE: | IgG2 |
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| SPECIES REACTIVITY: | - |
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| PREPARATION AND STORAGE: | - |
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Description
CD38, a type II transmembrane glycoproteins originally identified as an activation antigen of T and B cells. It is expressed on several leukocytes and early hematopoietic precursor cells. This molecules is also expressed in nonhematopoietic cells, including epithelial cells and astrocytes. CD38 is an ectoenzyme that catalyzes the synthesis of cyclic ADP ribose (a potent second messenger for Ca2+ release) and a receptor that initiates transmembrane signaling on engagement with its counterreceptor CD31 or with agonistic mAbs. The effects mediated by CD38 include the production of proinflammatory cytokines, proliferation, and protection from apoptosis in lymphocytes.
References
March S, Graupera M, Sarrias MR, Lozano F, Pizcueta P, Bosch J, and Engel P. Identification and Functional Characterization of the Hepatic Stellate Cell CD38 Cell Surface Molecule. AJP January 2007, Vol. 170, No. 1
Applications
IHC Techniques
| IHC Techniques |
Clone |
Dilution |
Antibody concentration |
Antigen retrieval method |
Visualization kit |
+/- control |
Protein localization |
Positivity in other species |
Protocol |
| Frozen tissue and cytospins |
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| Paraffin tissue |
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| Immunofluorescence |
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| CD38.14.27 |
1 ug |
|
|
|
/ Ig control |
rat CD38 |
|
|
Enlarge image
- Immunolocalization of CD38 in rat liver sections
- A–F: Liver sections of control rats (A and D), rats pretreated with LPS (550 ug/rat) (B and E) and cirrhotic rats with an advanced stage of fibrosis displaying prominent scars (C and D) were stained with an antibody against CD38 (CD38.14.27) (A–C) or with an Ig control (D–F). Liver sections were washed and incubated with an anti-mouse Cy-3-conjugated secondary antibody (red). Hepatocyte autofluorescence from hepatocytes is seen in green.
WB Techniques
| WB Techniques |
Clone |
Dilution |
Antibody concentration |
+/- control |
Expected MW |
Observed Mw |
Positivity in other species |
Protocol |
| Western Blotting |
|---|
| CD38.14.27 |
1 ug |
|
/ Ig control |
34.43 KD |
45 KD |
|
|
| Immunoprecipitation |
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Enlarge image
- Western blot analysis of the protein recognized by mAb CD38.14.27.
FLOW CYTOMETRY
| FLOW CYTOMETRY |
Clone |
Dilution |
+/- control |
Type of fluorocrom |
Protocol |
| CD38.14.27 |
1 ug |
/ Ig control |
|
|
Enlarge image
- Expression of CD38 on activated HSCs.
- HSCs were freshly isolated from control (top) and cirrhotic (bottom) rats. CD38+ cells were gated, and the intensity of CD38 expression was assessed by flow cytometry (FACS) using CD38.14.27 mAb. Fluorescence intensity is shown over a 3-decade log scale.
