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Pablo Engel

Name of the laboratory

UB: University of Barcelona
Immunology Unit
Department of Biomedical Sciences
Faculty of Medicine and Medical Sciences, University of Barcelona
08036 Barcelona, Spain
34 932 275 400 (ext.: 4010, 4011)

Members of the laboratory

Head of the Unit
Pablo Engel pengel@ub.edu
Ph.D. Student
Rebeca Gutiérrez Cozar rebeca.gutierrez@ub.edu
Ph.D. Student
Julen Merino julenmerino00@gmail.com
Technician
Javier Fernández Calles javierfdezcalles@gmail.com

Laboratory activity

The Immunology Unit at the University of Barcelona has focused it's studies on leukocyte cell-surface molecules. Monoclonal antibodies are essential tools for determining the expression and function of these molecules. Monoclonal antibodies produced in our Unit during the last 20 years have been used for research.

Dr. Engel has been the President of HCDM (Human Cell Differentiation Molecules) and the chair of the nomenclature and standardization committees for CD molecules and leukocytes of IUIS (International Union of Immunological Societies) 2010-2022. He has been Secretary General of EFIS (European Federation of Immunological Societies) until 2021.

Research activities

Characterization of the expression and function of the SLAM (Signaling Lymphocyte-Activation Molecule) family members: Our team has produced several antibodies against the human and mouse cell-surface molecules of the SLAM family including CD84, CD150 (SLAM), CD229 (Ly9), CD244 (2B4), SLAMF6 and SLAMF7. These reagents have been essential in establishing the expression and various functions of this new family of immunoreceptors.

Production and characterization of antibodies against P-selectin and CD69 using KO-mice: We have generated a large panel of monoclonal antibodies against the adhesion molecule P-selectin and CD69. These antibodies have been generated using knock-out KO-mice. A unique characteristic of these antibodies is that they are able to recognize epitopes preserved across multiple species. These antibodies have been used by a large group of collaborators to study the function of P-selectin and CD69 in vitro and in vivo in different animal model of disease.

Other antibodies: Antibodies against other cell-surface molecules recently produced in our lab include TNF receptor 2, human Syndecan-2, mouse GITRL, rat CD38. and several CMV proteins.

Production of antibodies against other cell-surface molecules: We are currently engaged in the production of antibodies that identify new markers for the diagnosis and treatment leukaemia and lymphoma.

Production of antibodies for the CAR-T cell Technology: During the last year we have produced antibdies agasit diferent tragets, including CD1d, CD22, CD84, CD229 among others.

Techniques available

  • Monoclonal antibody production in mice
  • Monoclonal antibody production in hamsters
  • Antibody characterization
  • Multi-colour flow cytometry
  • Immunization with transfected cells

Publications (2019-present)

  • Standardization of Workflow and Flow Cytometry Panels for Quantitative Expression Profiling of Surface Antigens on Blood Leukocyte Subsets: An HCDM CDMaps Initiative. Kužílková D, Puñet-Ortiz J, Aui PM, Fernández J, Fišer K, Engel P, van Zelm MC, Kalina T.Front Immunol. 2022 Feb 11;13:827898. doi: 10.3389/fimmu.2022.827898. eCollection 2022.PMID: 35222411
  • Decreased and Heterogeneous Neutralizing Antibody Responses Against RBD of SARS-CoV-2 Variants After mRNA Vaccination. Hernández-Luis P, Aguilar R, Pelegrin-Pérez J, Ruiz-Olalla G, García-Basteiro AL, Tortajada M, Moncunill G, Dobaño C, Angulo A, Engel P.Front Immunol. 2022 Apr 6;13:816389. doi: 10.3389/fimmu.2022.816389. eCollection 2022.PMID: 35464418
  • SIRPα - CD47 axis regulates dendritic cell-T cell interactions and TCR activation during T cell priming in spleen. Autio A, Wang H, Velázquez F, Newton G, Parkos CA, Engel P, Engelbertsen D, Lichtman AH, Luscinskas FW.PLoS One. 2022 Apr 12;17(4):e0266566. doi: 10.1371/journal.pone.0266566. eCollection 2022.PMID: 35413056
  • CD229 (Ly9) a Novel Biomarker for B-Cell Malignancies and Multiple Myeloma. Roncador G, Puñet-Ortiz J, Maestre L, Rodríguez-Lobato LG, Jiménez S, Reyes-García AI, García-González Á, García JF, Piris MÁ, Montes-Moreno S, Rodríguez-Justo M, Mena MP, Fernández de Larrea C, Engel P.Cancers (Basel). 2022 Apr 26;14(9):2154. doi: 10.3390/cancers14092154.PMID: 35565280
  • Discovery of the first PD-1 ligand encoded by a pathogen. Martínez-Vicente P, Poblador F, Leitner J, Farré D, Steinberger P, Engel P, Angulo A.Front Immunol. 2022 Sep 13;13:1007334. doi: 10.3389/fimmu.2022.1007334. eCollection 2022.PMID: 36177035
  • Experimental and genetic evidence for the impact of CD5 and CD6 expression and variation in inflammatory bowel disease. Casadó-Llombart S, Velasco-de Andrés M, Català C, Leyton-Pereira A, Gutiérrez-Cózar R, Suárez B, Armiger N, Carreras E, Esteller M, Ricart E, Ordás I, Gisbert JP, Chaparro M, Esteve M, Márquez L, Busquets D, Iglesias E, García-Planella E, Martín-Arranz MD, Lohmann J, Ayata CK, Niess JH, Engel P, Panés J, Salas A, Domènech E, Lozano F; ENEIDA Project of GETECCU.Front Immunol. 2022 Sep 21;13:966184. doi: 10.3389/fimmu.2022.966184. eCollection 2022.PMID: 36211446
  • CD6 deficiency impairs early immune response to bacterial sepsis. Català C, Velasco-de Andrés M, Leyton-Pereira A, Casadó-Llombart S, Sáez Moya M, Gutiérrez-Cózar R, García-Luna J, Consuegra-Fernández M, Isamat M, Aranda F, Martínez-Florensa M, Engel P, Mourglia-Ettlin G, Lozano F.iScience. 2022 Sep 5;25(10):105078. doi: 10.1016/j.isci.2022.105078. eCollection 2022 Oct 21.PMID: 36157587
  • RBD-Based ELISA and Luminex Predict Anti-SARS-CoV-2 Surrogate-Neutralizing Activity in Two Longitudinal Cohorts of German and Spanish Health Care Workers. Aguilar R, Li X, Crowell CS, Burrell T, Vidal M, Rubio R, Jiménez A, Hernández-Luis P, Hofmann D, Mijočević H, Jeske S, Christa C, D'Ippolito E, Lingor P, Knolle PA, Roggendorf H, Priller A, Yazici S, Carolis C, Mayor A, Schreiner P, Poppert H, Beyer H, Schambeck SE, Izquierdo L, Tortajada M, Angulo A, Soutschek E, Engel P, Garcia-Basteiro A, Busch DH, Moncunill G, Protzer U, Dobaño C, Gerhard M.Microbiol Spectr. 2023 Feb 14;11(1):e0316522. doi: 10.1128/spectrum.03165-22. Epub 2023 Jan 9.PMID: 36622140
  • Autophagy-mediated NCOR1 degradation is required for brown fat maturation and thermogenesis. Sabaté-Pérez A, Romero M, Sànchez-Fernàndez-de-Landa P, Carobbio S, Mouratidis M, Sala D, Engel P, Martínez-Cristóbal P, Villena JA, Virtue S, Vidal-Puig A, Palacín M, Testar X, Zorzano A.Autophagy. 2023 Mar;19(3):904-925. doi: 10.1080/15548627.2022.2111081. Epub 2022 Aug 25.PMID: 35947488
  • Cytomegalovirus restricts ICOSL expression on antigen-presenting cells disabling T cell co-stimulation and contributing to immune evasion. Angulo G, Zeleznjak J, Martínez-Vicente P, Puñet-Ortiz J, Hengel H, Messerle M, Oxenius A, Jonjic S, Krmpotić A, Engel P, Angulo A.Elife. 2021 Jan 18;10:e59350. doi: 10.7554/eLife.59350.PMID: 33459589 .
  • Autoimmune B Cell Repertoire in a Mouse Model of Sjögren's Syndrome. Sáez Moya M, Gutiérrez-Cózar R, Puñet-Ortiz J, Rodríguez de la Concepción ML, Blanco J, Carrillo J, Engel P.Front Immunol. 2021 Apr 23;12:666545. doi: 10.3389/fimmu.2021.666545. eCollection 2021.PMID: 33968069
  • Seven-month kinetics of SARS-CoV-2 antibodies and role of pre-existing antibodies to human coronaviruses. Ortega N, Ribes M, Vidal M, Rubio R, Aguilar R, Williams S, Barrios D, Alonso S, Hernández-Luis P, Mitchell RA, Jairoce C, Cruz A, Jimenez A, Santano R, Méndez S, Lamoglia M, Rosell N, Llupià A, Puyol L, Chi J, Melero NR, Parras D, Serra P, Pradenas E, Trinité B, Blanco J, Mayor A, Barroso S, Varela P, Vilella A, Trilla A, Santamaria P, Carolis C, Tortajada M, Izquierdo L, Angulo A, Engel P, García-Basteiro AL, Moncunill G, Dobaño C.Nat Commun. 2021 Aug 6;12(1):4740. doi: 10.1038/s41467-021-24979-9.PMID: 34362897 Free PMC article.
  • Characterization of Novel P-Selectin Targeted Complement Inhibitors in Murine Models of Hindlimb Injury and Transplantation. Zheng C, Ricci J, Zhang Q, Alawieh A, Yang X, Nadig S, He S, Engel P, Jin J, Atkinson C, Tomlinson S.Front Immunol. 2021 Nov 25;12:785229. doi: 10.3389/fimmu.2021.785229. eCollection 2021.PMID: 34899752
  • SOX11, CD70, and Treg cells configure the tumor-immune microenvironment of aggressive mantle cell lymphoma. Balsas P, Veloza L, Clot G, Sureda-Gómez M, Rodríguez ML, Masaoutis C, Frigola G, Navarro A, Beà S, Nadeu F, Giné E, López-Guillermo A, Martínez A, Ribera-Cortada I, Engel P, Quintanilla-Martínez L, Klapper W, Campo E, Amador V.Blood. 2021 Dec 2;138(22):2202-2215. doi: 10.1182/blood.2020010527.PMID: 34189576
  • Determinants of early antibody responses to COVID-19 mRNA vaccines in a cohort of exposed and naïve healthcare workers. Moncunill G, Aguilar R, Ribes M, Ortega N, Rubio R, Salmerón G, Molina MJ, Vidal M, Barrios D, Mitchell RA, Jiménez A, Castellana C, Hernández-Luis P, Rodó P, Méndez S, Llupià A, Puyol L, Rodrigo Melero N, Carolis C, Mayor A, Izquierdo L, Varela P, Trilla A, Vilella A, Barroso S, Angulo A, Engel P, Tortajada M, García-Basteiro AL, Dobaño C.EBioMedicine. 2022 Jan;75:103805. doi: 10.1016/j.ebiom.2021.103805. Epub 2022 Jan 12.PMID: 35032961