mAbs Against MOUSE Protein
Ly9 (recognizes Ly9.1 and Ly9.2)
| STATUS: | |
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| CONTACT INFORMATION: | Immunology Unit, Faculty of Medicine and Medical Sciences, University of Barcelona |
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| TYPE: | Mouse anti-mouse |
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| CLONE NAME: | mLy9.10.19 |
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| PROTEIN: | mouse Ly9 (CD229) |
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| ANTIGEN USED: | 300.19 pre-B cell line |
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| FUSION PARTNER: | NS1 cells |
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| ISOTYPE: | IgG1 (kappa) |
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| SPECIES REACTIVITY: | Mouse |
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| PREPARATION AND STORAGE: | Aliquot and store at 4ºC. Do not freeze |
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| APP RECOMMENDED: | Flow cytometry, ELISA, IP |
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| APP NO RECOMMENDED: | IHQ-paraffin, WB |
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| APP NO TESTED: | IF |
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Applications
WB Techniques
| WB Techniques |
Clone |
Dilution |
Antibody concentration |
+/- control |
Expected MW |
Observed Mw |
Positivity in other species |
Protocol |
| Western Blotting |
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| Immunoprecipitation |
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| mLy9.10.19 |
|
2 ug/ml |
/ Unrelated mAb |
110-120 |
120 |
Not done |
|
Enlarge image
- IP of mLy9.10.19
- Immunoprecipitation with 1 ml Supernetant, using biotin labeled 300.19 cells and Sepharose beads with protein G.
OTHERS
| OTHERS |
Title |
Description |
Protocol |
| test |
|
|
Enlarge image
- Thymus Ly9 KO mouse
- Flow cytometry with thymocytes of a Balb/c Ly9 KO mouse using anti-Mouse PE conjugated as secondary antibody
Enlarge image
- Thymus Ly9 Balb/c WT mouse
- Flow cytometry with thymocytes of a Balb/c Ly9 WT mouse using anti-Mouse PE conjugated as secondary antibody
Enlarge image
- Thymus Ly9 C57BL/6 WT mouse
- Flow cytometry with thymocytes of a C57BL/6 Ly9 WT mouse using anti-Mouse PE conjugated as secondary antibody
