mAbs Against MOUSE Protein
TRF1
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | rat anti mouse |
| CLONE NAME: | 572C |
| PROTEIN: | Mus musculus Telomeric repeat binding protein 1 |
| PROTEIN WEB: | http://www.uniprot.org/uniprot/P70371 |
| ANTIGEN USED: | mTRF1-His |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG2a |
| SPECIES REACTIVITY: | mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 572C | Undiluted supernatant | Supernatant | / | nuclear | ||||
| Paraffin tissue | |||||||||
| Immunofluorescence | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 572C | Undiluted supernatant | Supernatant | / | nuclear | Available | |||
Enlarge image
- Anti-TRF1 antibody (572C) in transfected cells.
- To confirm that 572C mAb recognized mouse TRF1 protein, immunohistochemistry on frozen cytospins preparations of mTRF1 expressed in Hek293T was performed. Hek293T-mTRF2-GFP was used as negative control. Anti-TRF1 Abcam antibody was used as positive control.
Enlarge image
- 572C mAb is able to detect mouse TRF1 protein by immunofluorescence.
- mTRF1- red
GFP - green
nuclei - blue
Enlarge image
- 572C mAb is able to detect mouse TRF1 protein in a mouse blastocyste.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 572C | Undiluted supernatant | Supernatant | / | 47kDa | 50kDa | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- 572C mAb is able to detect mouse TRF1 protein by WB
- Lanes:
Lane 1 IPS-GFP-mTRF1 homo (100ug) (+)
Lane 2 IPS-GFP-mTRF1 heter (100ug) (+/-)
Lane 3 IPS-sh mTRF1 (100ug) (-)
Lane 4 IPS-sh mTRF1 puro (100ug) (-)
Lane 5 Hek-mTRF1 (20ug) (+)
Lane 6 Hek-FGFR2 (20ug) (-)
Lane 7 NIH-3T3 (100ug) (+)
Enlarge image
- 572C mAb is able to detect mouse TRF1 protein by WB.
- Lanes:
Lane 1 Mefs TRF1 +/+ nuclear extract (30ug) (+)
Lane 2 Mefs TRF1 KO nuclear extract (30ug) (-)
Image kindly provided by Ianire Garrobo