EuroMAbNet: European Monoclonal Antibodies Network

Members

Alexander Taranin

Name of the laboratory

Members of the laboratory

Head

Alexander Taranin taranin@mcb.nsc.ru

Research Fellows

Konstantin Baranov baranov@mcb.nsc.ru

Nikolai Chikaev na_chik@mcb.nsc.ru

Anton Chikaevchikaev@mcb.nsc.ru

Andrey Gorchakovgorchakov@mcb.nsc.ru

Sergey Guselnikov sguselnikov@mcb.nsc.ru

Sergey Kulemzin skulemzin@mcb.nsc.ru

Ludmila Mechetina lucie@mcb.nsc.ru

Alexander Najakshin najakshin@mcb.nsc.ru

Evdokiya Reshetnikova ereshet@mcb.nsc.ru

Olga Volkova volkova@mcb.nsc.ru

Technicians

Anna Maerova maerova@mcb.nsc.ru

Zoya Chernishova z_chernishova@mcb.nsc.ru

Antonina Starikovskaya

Research activities

The Laboratory of Immunogenetics is one of the research groups that have discovered a new family of immunoreceptors , Fc Receptor Like (FCRL) molecules. We have produced mouse monoclonal and rabbit polyclonal antibodies against human FCRLA, FCRL1, FCRL4 and FCRL6 and polyclonal antibodies against mouse FCRLA. Using obtained antibodies we characterized structure and expression of these molecules in detail. We have also generated monoclonal antibodies against human SLAMF9, the least studied member of the SLAM family. Evolution of paired receptors of FcR, KIR and CD2 families is also under our study. Recently we have started production of llama VHH antibodies against therapeutically important human molecules.

Techniques available

• Production of murine monoclonal and rabbit polyclonal antibodies
• Production of llama antibodies
• Antibody characterization
• Cloning technique
• Flow cytometry
• Immunocytochemistry
• ELISA, Westen blot
• Antibody purification and labeling
• Recombinant protein expression in bacteria and mammalian and insect cells
• Phage display
• CAR technology

Publications (2019-present)

• Reshetnikova E, Guselnikov S, Volkova O, Baranov K, Taranin A, Mechetina L. B cell-specific protein FCRLA is expressed by plasmacytoid dendritic cells in humans. Cytometry B Clin Cytom, 2018, doi: 10.1002/cyto.b.21611
• Kulemzin SV, Gorchakov AA, Chikaev AN, Kuznetsova VV, Volkova OY, Matvienko DA, Petukhov AV, Zaritskey AY, Taranin AV. VEGFR2-specific FnCAR effectively redirects the cytotoxic activity of T cells and YT NK cells. (doi: 10.18632/oncotarget.24078) Oncotarget 9(10): 9021-9029, 2018
• Koval O, Volkova O, Kulemzin S, Gorchakov A, Tkachenko A, Nushtaeva A, Kuligina E, Richter V, Taranin A. NK-cell based delivery of anticancer therapeutics. (doi: 10.1093/annonc/mdx711.050) Annals Oncology 28(Suppl 11): 69P, 2017
• Gorchakov A, Kulemzin S, Kuznetsova V, Volkova O, Taranin A. FnCARs: a platform expanding the repertoire of proteins targetable with chimeric antigen receptors. Mol Therapy 25(5S1): 272, 2017
• Zaboronok AA, Byvaltsev VA, Kanygin VV, Iarullina AI, Kichigin AI, Taranin AV, Volkova OYu, Mechetina LV, Taskaev SYu, Muhamadiyarov RA, Zavyalov EL, Nakai K, Sato E, Yamamoto T, Mathis BJ, Matsumura A. Boron-neutron capture therapy in Russia: preclinical evaluation of efficacy and perspectives of its application in neuro-oncology. New Armenian Med J 11(1): 6-15, 2017
• Kulemzin SV, Kuznetsova VV, Mamonkin M, Taranin AV, Gorchakov AA. CAR T-cell therapy: Balance of efficacy and safety. Mol Biology 51(2): 237–250, 2017
• Kulemzin SV, Chikaev NA, Volkova OY, Kuznetsova VV, Taranin AV, Gorchakov AA. Modular lentiviral vector system for chimeric antigen receptor design optimization. Rus J Bioorg Chem 43(2): 107-114, 2017
• Kulemzin SV, Kuznetsova VV, Mamonkin M, Taranin AV, Gorchakov АA. Engineering chimeric antigen receptors. Acta Naturae 9(1): 6-14, 2017
• Sokratian A, Baranov K, Guselnikov S, Chikaev N, Volkova O, Mechetina L, Taranin A, Gorchakov A , Kulemzin S. Identification of the human SLAMF9-receptor complex. Tissue Antigens 85: 381-382, 2015
• Guselnikov SV, Grayfer L, De Jesús Andino F, Rogozin IB, Robert J, Taranin AV. Retention of duplicated ITAM-containing transmembrane signaling subunits in the tetraploid amphibian species Xenopus laevis. Dev Comp Immunol 53: 158-168, 2015
• Romanets-Korbut O, Najakshin AM, Yurchenko M, Malysheva TA, Kovalevska L, Shlapatska LM, Zozulya YA, Taranin AV, Horvat B, Sidorenko SP. Expression of CD150 in tumors of the central nervous system: identification of a novel isoform. PLoS One 10(2): e0118302, 2015
• Reguzova AY, Karpenko LI, Mechetina LV, Belyakov IM. Peptide–MHC multimer-based monitoring of CD8 T-cells in HIV-1 infection and AIDS 5 vaccine development. Expert Rev Vaccines 14: 69-84, 2015
• Volkova O, Guselnikov S, Mechetina L, Chikaev N, Baranov K, Kulemzin S, Reshetnikova E, Najakshin A, Taranin A. Development and characterization of domain-specific monoclonal antibodies produced against human SLAMF9. Monoclon Antib Immunodiagn Immunother 33: 209-214, 2014
• Kulemzin S, Chikaev N, Volkova O, Reshetnikova E, Taranin A, Najakshin A, Mechetina L. Characterization of human FCRLA isoforms. Immunol Lett 152: 153-158, 2013
• Reshetnikova ES, Mechetina LV, Volkova OY, Guselnikov SV, Chikaev NA, Kövesdi D, Alabyev B, Sármay G, Burrows PD, Najakshin AM, Taranin AV (2012). Differential expression of FCRLA in naïve and activated mouse B cells. Cell Immunol 272(2):182-92.
• Baranov KO, Volkova OY, Mechetina LV, Chikaev NA, Reshetnikova ES, Nikulina GM, Taranin AV, Naiakshin AM (2012). [Expression of human B-cell specific receptor FCRL1 in normal individuals and in patients with autoimmune diseases].Mol Biol (Mosk) 46(3):500-7. Russian.
• Reshetnikova ES, Mechetina LV, Taranin AV (2012). No title. Cytometry Part B - Clinical Cytometry 82B(4): O10-O10, 2012
• Reshetnikova ES, Mechetina LV, Taranin AV (2012). Differential expression of FCRLA in B lymphocytes in human tonsils. Cytometry Part B - Clinical Cytometry 82B(4): O17-O17.
• Karpenko LI, Danilenko AV, Bazhan SI, Danilenko ED, Sysoeva GM, Kaplina ON, Volkova OY, Oreshkova SF, Ilyichev AA (2012). Attenuated Salmonella enteritidis E23 as a vehicle for the rectal delivery of DNA vaccine coding for HIV-1 polyepitope CTL immunogen. Microb Biotechnol 5(2):241-50.
• Santiago T, Kulemzin SV, Reshetnikova ES, Chikaev NA, Volkova OY, Mechetina LV, Zhao M, Davis RS, Taranin AV, Najakshin AM, Hendershot LM, Burrows PD (2011). FCRLA is a resident endoplasmic reticulum protein that associates with intracellular Igs, IgM, IgG and IgA. Int Immunol 23(1):43-53.
• Kulemzin SV, Zamoshnikova AY, Yurchenko MY, Vitak NY, Najakshin AM, Fayngerts SA, Chikaev NA, Reshetnikova ES, Kashirina NM, Peclo MM, Rutkevich PN, Shevelev AY, Yanushevskaya EV, Baranov KO, Mamonkin M, Vlasik TN, Sidorenko SP, Taranin AV, Mechetina LV (2011). FCRL6 receptor: Expression and associated proteins. Immunol Lett 134: 174-182.
• Guselnikov SV, Laktionov PP, Najakshin AM, Baranov KO, Taranin AV (2011). Expansion and diversification of the signaling capabilities of the CD2/SLAM family in Xenopodinae amphibians. Immunogenetics 63: 679-689.
• Karpenko LI, Mechetina LV, Reguzova AYu (2011). MHC-multimers and their application in studies of antiviral immune response. J Microbiol Epidemiol Immunobiol N2: 112-119.
• Guselnikov SV, Reshetnikova ES, Najakshin AM, Mechetina LV, Robert J, Taranin AV (2010). The amphibians Xenopus laevis and Silurana tropicalis possess a family of activating KIR-related Immunoglobulin-like receptors. Dev Comp Immunol 34: 308-315.
• Guselnikov SV, Ramanayake T, Robert J, Taranin AV (2009). Diversity of the FcR- and KIR-related genes in an amphibian Xenopus. Front Biosci 14: 130-140.
• Goyos A, Ohta Y, Guselnikov S, Robert J (2009). Novel nonclassical MHC class Ib genes associated with CD8 T-cell development and thymic tumors. Mol Immunol 46: 1775-1786.
• Guselnikov SV, Ramanayake T, Erilova AY, Mechetina LV, Najakshin AM, Robert J, Taranin AV (2008). The Xenopus FcR family demonstrates continually high diversification of paired receptors in vertebrate evolution. BMC Evol Biol 8: 148.
• Baranov K, Volkova O, Chikaev N, Mechetina L, Laktionov P, Najakshin A, Taranin A (2008). A direct antigen-binding assay for detection of antibodies against native epitopes using alkaline phosphatase-tagged proteins. J Immunol Methods. 2008 Mar 20;332(1-2):73-81.