Members
Friedrich Koch-Nolte
Name of the laboratory
- Laboratory of Molecular Immunology
- Institute of Immunology
- University Medical Center Hamburg-Eppendorf
- Martinistr. 42
- 20246 Hamburg, Germany
- xx49-40-741053612
Members of the laboratory
- Unit Leader
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Friedrich Koch-Nolte nolte@uke.de
- Research Fellows
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Stephan Menzel s.menzel@uke.de
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Nicole Schwarz n.schwarz@uke.de
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Thomas Eden t.eden@uke.de
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Anna Eichhoff a.eichhoff@uke.de
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Mirjam Loew m.loew@uke.de
- Technicians
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Fabienne Seyfried f.seyfried@uke.de
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Gudrun Dubberke gdubberk@uke.de
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Birte Albrecht b.albrecht@uke.de
- Ph.D. Students
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Carolina Pinto c.pinto@uke.de
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Thomas Eden t.eden@uke.de
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Anna Eichhoff a.eichhoff@uke.de
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Intissar Chachbani i.chachbani@uke.de
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Tobias Schaefer to.schaefer@uke.de
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Julia Hambach j.hambach@uke.de
Research activities
The focus of our laboratory is on the molecular characterization of lymphocyte membrane proteins, in particular receptors and enzymes involved in signaling by extracellular nucleotides. We generate monoclonal antibodies and nanobodies - single domain antibodies derived from camelid heavy chain antibodies - as new research and therapeutic tools. We are interested in ADP-ribosylation as a pathogenic mechanism of bacterial toxins and as a reversible posttranslational modification regulating protein functions. Using genetic immunization and antibody engineering, we strive to develop new tools for combating infections and for treating diseases of the immune system. Many of our monoclonal and recombinant antibodies are commercialized via MediGate GmbH, a 100% subsidy of the University Medical Center Hamburg-Eppendorf.
Techniques available
- Monoclonal antibody production in rats and mice
- Polyclonal antibody production
- Genetic immunization
- Nanobody production from llamas and dromedaries
- Recombinant antibody production in HEK cells
- Cloning techniques
- Antibody characterization and conjugation
- Mycoplasma testing
- Flow cytometry, ELISA, immunofluorescence microscopy, etc.
Publications (2019-present)
- The ABCD database: a repository for chemically defined antibodies. Lima WC, Gasteiger E, Marcatili P, Duek P, Bairoch A, Cosson P. Nucleic Acids Res. 2019 Aug 14. pii: gkz714. doi: 10.1093/nar/gkz714. [Epub ahead of print] PMID:31410491
Reviews
- Stortelers C, Pinto-Espinoza C, Van Hoorick D, Koch-Nolte F. (2018) Modulating ion channel function with antibodies and nanobodies. Curr Opin Immunol. 52:18-26.
- Bannas P, Hambach J, Koch-Nolte F. (2017) Nanobodies and nanobody-based human heavy chain antibodies as antitumor therapeutics. Front Immunol. 8:1603.
- Rissiek B, Koch-Nolte F, Magnus T. (2014) Nanobodies as modulators of inflammation: potential applications for acute brain injury. Front Cell Neurosci. 8:344.
- Menzel S, Rissiek B, Haag F, Goldbaum F, Koch-Nolte F. (2013) The art of blocking ARTs: Nanobodies as experimental and therapeutic tools to block mammalian and toxin ADP-ribosyltransferases. FEBS J. 280:3543-50.
- Wesolowski J, Alzogaray V, Reyelt J, Unger M, Juarez K, Urrutia M, Cauerhff A, Danquah W, Rissiek B, Scheuplein F, Schwarz N, Adriouch S, Boyer O, Seman M, Licea A, Serreze DV, Goldbaum FA, Haag F, Koch-Nolte F (2009) Single domain antibodies: promising experimental and therapeutic tools in infection and immunity. (review) Med Microbiol Immunol 198:157-74.
- Koch-Nolte F, Haag F, Guse AH, Lund F, Ziegler M. (2009) Emerging roles of NAD+ and its metabolites in cell signaling. Sci Signal. 10:mr1.
- Koch-Nolte F, Kernstock S, Mueller-Dieckmann C, Weiss MS, Haag F. (2008) Mammalian ADP-ribosyltransferases and ADP-ribosylhydrolases. Front Biosci. 2008 May 1;13:6716-29.